Project

Part:BBa_K1230013:Design

Designed by: Andrés Ferriño Iriarte   Group: iGEM13_Ciencias-UNAM   (2013-09-25)


araC-pBad-rbs-LL37-rbs-GFP-pLacI-rbs-MarA Generator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1164
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 999
    Illegal AgeI site found at 2632
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2031
    Illegal SapI site found at 981


Design Notes

In order for this device to work, it has to be transformed into Top 10 cells, the strain for arabinose induction, in which LacI promoter is constitutive.


Source

GenScript 2013 DNA distribution

References